islet extraction machine

A machine learning approach to predict pancreatic islet …

A machine learning approach to predict pancreatic islet grafts rejection versus tolerance. PLoS One. 2020 Nov 5;15 (11):e0241925. doi: 10.1371/journal.pone.0241925. eCollection 2020.

Islet cell transplantation

Islet transplantation is the transplantation of isolated islets from a donor pancreas into another person. It is an experimental treatment for type 1 diabetes mellitus. Once transplanted, the islets begin to produce insulin, actively regulating the level of glucose in the blood. ...

A Simple High Efficiency Protocol for Pancreatic Islet …

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice J Vis Exp. 2019 Aug 30;(150):10.3791/57048. doi: 10.3791/57048. Authors Daniel Villarreal 1, Geetali Pradhan 2, Chia-Shan Wu 3, Clinton D Allred, ...

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The Evaluation of Islet Purification Methods That Use Large …

 · The most common method of islet purification is density gradient centrifugation using a COBE 2991 cell processor. However, this method can damage islets mechanically through its high shearing force. We recently reported that a new purification method using large plastic bottles effectively achieves a high yield of islets from the porcine pancreas.

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Postnatal Ontogenesis of the Islet Circadian Clock Plays a …

Islet RNA Extraction, Quantitative Real-time PCR, and Affymetrix GeneChip Isolated islets were washed with PBS to remove RPMI medium and immediately transferred to RNeasy Lysis Buffer RLT (Qiagen, Valencia, CA) and stored at −80°C for subsequent RNA isolation.

Islet cell antigens. Extraction studies and ELISA analysis.

Islet cell antigens. Extraction studies and ELISA analysis. Moncayo R (1), Kirsch K, Pasquarello T, Pfeiffer EF. (1)Department of Internal Medicine, University of Ulm, Federal Republic of Germany. Whole human and bovine pancreases were extracted in 20 mM Tris-HCl buffer without detergents and fractionated by high-speed centrifugation.

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Enhanced function of pancreatic islets co-encapsulated …

 · Islet RNA extraction and qRT-PCR Analysis of mRNA levels of islet functional genes (insulin I, insulin II, glucagon, somatostatin, PDX-1) and de-differentiation genes (cytokeratin 19, vimentin, E-cadherin) were assessed with real time qRT-PCR.

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The role of gut microbiota and amino metabolism in the …

 · The hypoglycemic effect and the improvement in islet β-cell function depend on the changes in ... put 20 μL of each hole in the sample tank and mixed that into the QC sample of the machine, ...

Islet isolation from juvenile porcine pancreas after 24-h …

Pancreas procurement for islet isolation and transplantation is limited by concerns for the detrimental effects of postmortem ischemia. Hypothermic machine perfusion (HMP) preservation technology has had a major impact in circumventing ischemic injury in clinical

A rapid, efficient, and economic device and method for the …

 · Rapid, efficient, and economic method for the isolation and purification of islets has been pursued by numerous islet-related researchers. In this study, we compared the advantages and disadvantages of our developed patented method with those of commonly used conventional methods (Ficoll-400, 1077, and handpicking methods). Cell viability was assayed using Trypan blue, cell purity …

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A supervised learning framework for pancreatic islet …

 · Islet cell quantification and function is important for developing novel therapeutic interventions for diabetes. Existing methods of pancreatic islet segmentation in …

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Islet Transplantation | SpringerLink

Islet Cell Acinar Cell Diabetic State Islet Transplantation Islet Tissue These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Development of an automated computer-controlled islet …

Before clinical islet transplantation can become an effective and reliable treatment for type 1 diabetic patients, there must be significant improvements in the methods employed for the isolation of islets of Langerhans. We have developed an automated cell extraction

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Islet Surface Modification with Urokinase through DNA …

Cell Labeling with Cy3 through DNA Hybridization for Assessing Neural Stem Cells Survival and Differentiation. Bioconjugate Chemistry 2018, 29 (11), 3561-3570. DOI: 10.1021/acs.bioconjchem.8b00533. Peter Y. Li, Zhiyuan Fan, Hao Cheng. Cell Membrane Bioconjugation and Membrane-Derived Nanomaterials for Immunotherapy.

Multi-Center Analysis of Novel and Established Variables …

improving islet extraction and transplantation rates which may ultimately lead to superior post-transplant ... Vazquez A, Brassil J. Twenty-four hour hypothermic machine perfusion preservation of porcine pancreas facilitates processing for islet isolation. ] [] ...

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HISTORY OF MEDICINE: Extraction of insulin from islet of …

 · Extraction of insulin from islet of Langerhans The term of "diabetes" was coined by the Greek Aretaeus in the second century AD. In 1776, the Liverpool physician, natural philosopher and experimental physiologist Matthew Dobson (1732 or 1735–1784) discovered that urine of diabetic patients is sweet because of excess in sugar.

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Standardization of islet isolation outcome

Home Browse by Title Periodicals Expert Systems with Applications: An International Journal Vol. 38, No. 4 Standardization of islet isolation outcome - A new automatic system to determine pancreatic islet …

Islet Cell Transplantation | Encyclopedia

The extraction process uses specialized enzymes to isolate the islet cells from the other cell types found in the pancreas. During the infusion process, the cells travel through the portal vein and become lodged in the capillaries of the liver, where they remain to produce insulin as they normally would in …

US4868121A

islet intact solution Prior art date 1985-02-07 Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Expired - Lifetime

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Genolution, headquartered in Seoul, South Korea, is a manufacturer of nucleic acid extraction equipment and supplies, including extraction kits for molecular biology and life sciences. Hain Lifescience is a manufacturer and distributor of in-vitro diagnostics including molecular genetic test systems and technical instruments for the detection of dangerous pathogens or hereditary diseases.

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Standardization of islet isolation outcome – A new …

 · In the proposed study, we have developed software capable of objectively evaluating islet numbers and other viability variables by image analysis. This software is based on image processing and feature extraction algorithms for recognition of the area of interest.